DNA sequencing is a process of determining nucleic acid sequence (adenine, guanine, cytosine, thymine) in a piece of DNA.

DNASEQ@INFORMM is offering DNA sequencing service for PCR products (purified and un-purified) based on Sanger method in an automated system, ABI 3130xl Genetic Analyzer (GA). The system uses fluorescently labelled terminators, capillary electrophoresis separation and automated laser signal detection for nucleotide sequence detection. Please find below fee structure and contacts for DNASEQ@INFORMM.

*RXN = Reaction

Remarks:

• For UNPURIFIED PRODUCT, only accept PCR product sample.
• For PURIFIED PRODUCT, gel image must be included.
• Reverse and Forward primers need to be labelled and submitted in separate tube.
• Forward and Reverse reaction will be charged individually (per reaction).
• Other requirement shall be further communicated to customer by related personnel.

Enquiries Please Contact:

DNASEQ@INFORMM
Institute of Research in Molecular Medicine (INFORMM)
Universiti Sains Malaysia,
Kampus Kesihatan 16150 Kubang Kerian, Kelantan

Mawaddah binti Mohd Azlan (Technical & Service Personnel)
Elis Rosliza Mohd Adzmi (Technical & Service Personnel)
Tel: +609-767 2411 / 2406 / 2404 / 2402 / 2433
Email: mawaddahmohdazlan@usm.my or elis@usm.my

DNA sequencing is a process of determining nucleic acid sequence (adenine, guanine, cytosine, thymine) in a piece of DNA.

DNASEQ@INFORMM is offering DNA sequencing service for PCR products (purified and un-purified) based on Sanger method in an automated system, ABI 3130xl Genetic Analyzer (GA). The system uses fluorescently labelled terminators, capillary electrophoresis separation and automated laser signal detection for nucleotide sequence detection. Please find below fee structure and contacts for DNASEQ@INFORMM.

*RXN = Reaction

Remarks:

• For UNPURIFIED PRODUCT, only accept PCR product sample.
• For PURIFIED PRODUCT, gel image must be included.
• Reverse and Forward primers need to be labelled and submitted in separate tube.
• Forward and Reverse reaction will be charged individually (per reaction).
• Other requirement shall be further communicated to customer by related personnel.

Enquiries Please Contact:

DNASEQ@INFORMM
Institute of Research in Molecular Medicine (INFORMM)
Universiti Sains Malaysia,
Kampus Kesihatan 16150 Kubang Kerian, Kelantan

Mawaddah binti Mohd Azlan (Technical & Service Personnel)
Elis Rosliza Mohd Adzmi (Technical & Service Personnel)
Tel: +609-767 2411 / 2406 / 2404 / 2402 / 2433
Email: mawaddahmohdazlan@usm.my or elis@usm.my

Pyrosequencing is a type of DNA sequencing based on the “sequencing by synthesis” method. Unlike traditional Sanger sequencing, Pyrosequencing does not require fluorescence dye labelling or gel electrophoresis, while allowing quantitative, real time detection of sequencing events. Pyro@INFORMM is offering a pyrosequencing service for SNP and mutational analysis as well as quantitative analysis of CpG methylation. Please find below the details of the packages offered by us.

SERVICE CHARGES

No.	Package	Price (RM)
1.	Primer design using “Pyromark Assay Design 2.0” software Note: Primer design by users	FOC
2.	Machine handling Note: i. Consumables and reagent provided by user         ii. Capacity: Maximum 88 reactions/ run	RM 70.00/day Up to 3 run/day
3.	Full service Note: Consumables and reagent provided by INFORMM	RM 200.00/reaction

SERVICE ORDERING PROCEDURE

• Contact INFORMM person-in-charge for booking slot
• Submit Order Request Form
• Prepare samples (See below for details)
• Submit samples and sequencing primer(s) to person-in-charge
• Attach Pyrosequencing Sample Submission Form

SAMPLE PREPARATION GUIDELINES

• Prepare biotin-labelled PCR fragments (Sequence to be analyzed) according to the assay design
• The PCR primer that is complementary to the sequencing primer MUST be HPLC-purified and biotin-labelled
• Use no more than 0.2uM PCR primers with high number of PCR cycle to deplete excess biotin-labelled primers. Excess biotin-labelled primers will decrease assay efficiency
• The PCR products MUST be specific and clean (i.e. without primer dimers and non-specific products)
• 2-5 pmoles of PCR product are required per reaction. We strongly recommend quantifying of the PCR products by running a DNA Mass ladder in the same agarose gel

SAMPLE SUBMISSION

• Prepare 2-5 pmoles of PCR products in a normalized volume between 20-50 ul in a 96-well plate.
• Load maximum 88 samples per plate in columns 1 to 11 only.
• Seal the 96-well plate with adhesive film securely.
• Label the plate clearly with your name and date.
• Dilute the sequencing primer to 20uM and prepare at least 1.5ul per sample in 1.5 ml microtube.
• Label the tube clearly with primer name and date.
• Put the plate and tube(s) in clear plastic bag.

CONTACT PERSON

Mr. Muhammad Fazli Khalid (fazlikhalid@usm.my; +609-7672428)
Miss Basyirah Ghazali (basyirah@usm.my; +609-7672414)

Administration office Tel: +609-7672403

INFORMM, USM Health Campus is offering a facility rental for prototype development on lateral flow assay. The facility is fully equipped from dispensing to packing of the product. Please find below the details of the packages offered by us.

FACILITY FEATURES

• Dry room (Relative humidity: 10%-20%)
• Reagent dispenser (BioDot XYZ3050)
• Dry cabinet
• Strip cutter
• Heat sealer

SERVICE CHARGES

No.	Ruby Package	Price (RM)
1.	Day 1: Dispensing antigen onto membrane card Facility: Dry room Equipment: Reagent dispenser Day 2: Assembly, cutting and packaging Facility: Dry room Equipment: Strip cutter & Heat sealer Note: INFORMM will provide pouches and desiccant up to 500 pcs.	RM2962.70

.

No.	Ruby Plus Package	Price (RM)
1.	Day 1: Dispensing antigen onto membrane card Facility: Dry room Equipment: Reagent dispenser Day 2: Blocking & drying membrane Facility: Dry room/ dry cabinet Day 3: Assembly, cutting and packaging Facility: Dry room Equipment: Strip cutter & Heat sealer Note: INFORMM will provide pouches and desiccant up to 500 pcs.	RM3651.70

SERVICE ORDERING PROCEDURE

• Contact INFORMM person-in-charge for booking slot
• Submit Order Request Form

 INFORMM RESPONSIBILITIES

• Assist user(s) to set-up the facilities/room.
• Assist user(s) to handling the equipment.
• Advice user(s) in the prototype development, if requested by user(s).

USERS RESPONSIBILITIES

• Provide all the raw materials including sample pad, nitrocellulose membrane, conjugate pad, absorbance pad and backing card.
• Provide at least 700 μL of antigen/antibody for dispensing.

CONTACT PERSON

Mr. Muhammad Fazli Khalid (fazlikhalid@usm.my; Tel: +609-764 2428)
Mr. Muhamad Nuramin Ahmad (nuramin@usm.my; Tel: +609-764 2423)
Administration office: Phone: +609-764 2403