Rahmah Noordin
Name
Rahmah Noordin
Current Position
Honorary Professor
rahmah8485@gmail.com
Office Telephone
+604-6534854
Personal Lab Website
Qualifications
Ph.D. Universiti Sains Malaysia, 1993)
M. Sc Indiana State University, Terre Haute Indiana, USA, 1985
B. Sc. (Magna Cum Laude) Indiana State University, USA, 1983
Affiliations
Fellow of Academy of Sciences Malaysia (F.A.Sc.) since 2011
Life member of Malaysian Society of Parasitology & Tropical Medicine, since 1997
Member of American Society of Tropical Medicine and Hygiene, 2009 to 2016 (Member ID: 33674)
Research Interests
Biomarker discovery for parasitic diseases, recombinant proteins; protein expression and purification, immunoassay development and evaluation, lateral flow rapid test
Research Overview 1
Rapid diagnostic tests lymhatic filariasis
There are two types of lymhatic filariasis(LF) namely brugian and bancroftian LF, the latter comprised 90% of the global disease burden. The patented BmR1 recombinant protein was discovered via a targeted immunoscreening of a Brugia malayi cDNA library. It was then used for development of a lateral flow cassette test called Brugia Rapid for detection of B. malayi and B. timori and has been licensed to Reszon Diagnostics International Sdn. Bhd. The test had undergone extensive multicenter evaluations/validations in the laboratory and in the field, as well as under operational conditions. Brugia Rapid has been recognized by the World Health Organization (WHO) for use as a diagnostic tool in brugian LF countries as part of the Global Programme for Elimination of Lymphatic Countries (GPELF). PanLF Rapid, also commercialized by Reszon, is an extension of Brugia Rapid, and contains an additional infection marker called BmSXP that is sensitive for detection of bancroftian filariasis caused by Wuchereria bancrofti. It is meant for use in brugian LF countries that has some areas of possible co-infection with bancroftian filariasis. The Ministry of Health Malaysia is using PanLF in its LF elimination program. BLF Rapid is a prototype rapid test kit for highly sensitive detection of bancroftian filariasis. It has been evaluated in USA (at CDC Atlanta and University of Washington, St Louis), India and Malaysia with excellent results.
Research Overview 2
Rapid disgnostic tests for amoebiasis
Amoebiasis is caused by the protozoan parasite, Entamoeba histolytica that causes intestinal and extraintestinal amoebiasis. Due to the lack of a rapid test for the latter, we embarked on the analysis of excretory-secretory antigens (ESA) of E. histolytica which led to the discovery of pyruvate phosphate dikinase (PPDK) as a good diagnostic marker for amoebic liver abscess, and a patent has been granted for this finding. The recombinant form of PPDK was produced and a lateral flow rapid IgG4 test was developed which is called XEh Rapid. The prototype test has undergone multicenter national and international evaluations with positive outcomes. In addition, we have developed a proof of concept rapid antigen detection test for intestinal amoebiasis using antibodies to rPPDK and ESA. Although the test showed better sensitivity compared to the major antigen detection ELISA in the market, further work will be undertaken to increase the sensitivity of our test, and it will be followed by multicenter evaluations.
Research Overview 3
Rapid diagnostic test for other parasitic diseases
We are also working on developing and refining several other lateral flow rapid tests for parasitic infections. Tc Rapid is a prototype rapid test for detection of toxocariasis, and it uses several Toxocara canis recombinant proteins, one of them has been patented. Since T. cati has been implicated in some cases of toxocariasis, especially in certain human populations where cats are more favoured than dogs, we are now working on improving Tc Rapid by incorporating a T.cati recombinant protein.
Another recombinant antigen-based lateral flow rapid test, Strongy Rapid, has been developed to detect strongyloidiasis and is now at an advanced stage of development. Due to the phenomenon of autoinfection, this parasite can survive for many years in the body. Thus immunosuppressed patients who are living or used to live in endemic areas should be tested for this infection to prevent potentially fatal outcomes due to exacerbation of the infection.
We have also developed a lateral flow rapid test (Hyd Rapid) using a unique recombinant AgB protein for specific IgG4 detection of hydatid disease. An antigen detection test for hydatid disease is also being developed using antibodies to various forms of Echinococcus granulosus antigens. In addition, an early prototype rapid test for IgM detection of toxoplasmosis has been developed using two recombinant proteins, one of them has been filed for patent.
Selected Publications
1.Wong WK, Foo PC, Olivos-Garcia A, Noordin R, Mohamed Z, Othman N, Few LL, Lim BH. Parallel ELISAs using crude soluble antigen and excretory-secretory antigen for improved serodiagnosis of amoebic liver abscess. Acta Trop. 2017 May 12;172:208-212. doi: 10.1016/j.actatropica.2017.05.017.
2: Sahimin N, Lim YAL, Ariffin F, Behnke JM, Basáñez MG, Walker M, Lewis JW, Noordin R, Abdullah KA, Mohd Zain SN. Socio-demographic determinants of Toxoplasma gondii seroprevalence in migrant workers of Peninsular Malaysia. Parasit Vectors. 2017 May 15;10(1):238. doi: 10.1186/s13071-017-2167-8.
3: Saidin S, Yunus MH, Othman N, Lim YA, Mohamed Z, Zakaria NZ, Noordin R. Development and initial evaluation of a lateral flow dipstick test for antigen detection of Entamoeba histolytica in stool sample. Pathog Glob Health. 2017 May;111(3):128-136. doi: 10.1080/20477724.2017.1300421.
4: Mirzadeh A, Saadatnia G, Golkar M, Babaie J, Noordin R. Production of refolded Toxoplasma gondii recombinant SAG1-related sequence 3 (SRS3) and its use for serodiagnosis of human toxoplasmosis. Protein Expr Purif. 2017 May;133:66-74. doi: 10.1016/j.pep.2017.03.001.
5: Ujang JA, Kwan SH, Ismail MN, Lim BH, Noordin R, Othman N. Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC-ESI-MS/MS and LC-MALDI-TOF/TOF. Clin Proteomics. 2016 Nov 22;13:33. eCollection 2016.
6: Teh AY, Amerizadeh A, Osman S, Yunus MH, Noordin R. Identification, production and assessment of two Toxoplasma gondii recombinant proteins for use in a Toxoplasma IgG avidity assay. Pathog Glob Health. 2016 Oct -Dec;110(7-8):277-286. .
7: Rahumatullah A, Khoo BY, Noordin R. Development of triplex real-time PCR and detection of Toxoplasma gondii DNA in infected mice tissues and spiked human samples. Trop Biomed. 2015 Jun;32(2):376-85.
8: Zainudin NS, Othman N, Muhi J, Abdu Sani AA, Noordin R. Mature Erythrocyte Surface Antigen Protein Identified in the Serum of Plasmodium falciparum-Infected Patients. Am J Trop Med Hyg. 2015 Dec;93(6):1268-73. doi: 10.4269/ajtmh.15-0333.
9: Zahabiun F, Sadjjadi SM, Yunus MH, Rahumatullah A, Moghaddam MH, Saidin S,Noordin R. Production of Toxocara cati TES-120 Recombinant Antigen and Comparison with its T. canis Homolog for Serodiagnosis of Toxocariasis. Am J Trop Med Hyg.
2015 Aug;93(2):319-25. doi: 10.4269/ajtmh.15-0190.
10: Chang CH, Riazi M, Yunus MH, Osman S, Noordin R. Limited diagnostic value of two commercial rapid tests for acute leptospirosis detection in Malaysia. Diagn Microbiol Infect Dis Dec;80(4):278-81. doi: 10.1016/j.diagmicrobio.2014.08.012.
11: Khalilpour A, Sadjjadi SM, Moghadam ZK, Yunus MH, Zakaria ND, Osman S, Noordin R. Lateral flow test using Echinococcus granulosus native antigen B and comparison of IgG and IgG4 dipsticks for detection of human cystic echinococcosis. Am J Trop Med Hyg. 2014 Nov;91(5):994-9. doi:10.4269/ajtmh.14-0170.
12: Huat LB, Garcia AO, Ning TZ, Kin WW, Noordin R, Azham SS, Jie LZ, Ching GC, Chong FP, Dam PC. Entamoeba histolytica acetyl-CoA synthetase: biomarker of acute amoebic liver abscess. Asian Pac J Trop Biomed. 2014 Jun;4(6):446-50. doi:10.12980/APJTB.4.2014C1169.
13: Zueter AM, Mohamed Z, Abdullah AD, Mohamad N, Arifin N, Othman N, Noordin R. Detection of Strongyloides stercoralis infection among cancer patients in a major hospital in Kelantan, Malaysia. Singapore Med J. 2014 Jul;55(7):367-71.
14: Saidin S, Yunus MH, Zakaria ND, Razak KA, Huat LB, Othman N, Noordin R. Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infect Dis. 2014 Apr 4;14:182. doi: 10.1186/1471-2334-14-182. Erratum in: BMC Infect Dis. 2014;14:533.
15: Supali T, Djuardi Y, Bradley M, Noordin R, Rückert P, Fischer PU. Impact of six rounds of mass drug administration on Brugian filariasis and soil-transmitted helminth infections in eastern Indonesia. PLoS Negl Trop Dis. 2013 Dec 12;7(12):e2586. doi: 10.1371/journal.pntd.0002586. eCollection 2013.
16: Chu BK, Deming M, Biritwum NK, Bougma WR, Dorkenoo AM, El-Setouhy M, Fischer PU, Gass K, Gonzalez de Peña M, Mercado-Hernandez L, Kyelem D, Lammie PJ, Flueckiger RM, Mwingira UJ, Noordin R, Offei Owusu I, Ottesen EA, Pavluck A, Pilotte N, Rao RU, Samarasekera D, Schmaedick MA, Settinayake S, Simonsen PE, Supali T, Taleo F, Torres M, Weil GJ, Won KY. Transmission assessment surveys (TAS) to define endpoints for lymphatic filariasis mass drug administration: a multicenter evaluation. PLoS Negl Trop Dis. 2013 Dec 5;7(12):e2584. doi: 10.1371/journal.pntd.0002584. eCollection 2013.
17: Othman N, Zainudin NS, Mohamed Z, Yahya MM, Leow VM, Noordin R. Protein expression in sera of patients with amoebic liver abscess (ALA): potential use of haptoglobin as a surrogate disease marker. Trop Biomed. 2013 Jun;30(2):257-66.
18: Amerizadeh A, Khoo BY, Teh AY, Golkar M, Abdul Karim IZ, Osman S, Yunus MH, Noordin R. Identification and real-time expression analysis of selected Toxoplasma gondii in-vivo induced antigens recognized by IgG and IgM in sera of acute toxoplasmosis patients. BMC Infect Dis. 2013 Jun 24;13:287. doi: 10.1186/1471-2334-13-287.
20: Othman N, Mohamed Z, Yahya MM, Leow VM, Lim BH, Noordin R. Entamoeba histolytica antigenic protein detected in pus aspirates from patients with amoebic liver abscess. Exp Parasitol. 2013 Aug;134(4):504-10. doi: 10.1016/j.exppara.2013.05.001.
21: Puvanesuaran VR, Noordin R, Balakrishnan V. Isolation and genotyping of Toxoplasma gondii from free-range ducks in Malaysia. Avian Dis. 2013 Mar;57(1):128-32.
22: Puvanesuaran VR, Noordin R, Balakrishnan V. Genotyping of Toxoplasma gondii isolates from wild boars in Peninsular Malaysia. PLoS One. 2013 Apr 17;8(4):e61730. doi:10.1371/journal.pone.0061730.
23: Deshpande PS, Kotresha D, Noordin R, Yunus MH, Saadatnia G, Golkar M, Osman S, Karim IZ, Ghaffarifar F. IgG avidity Western blot using Toxoplasma gondii rGRA-7 cloned from nucleotides 39-711 for serodiagnosis of acute toxoplasmosis. Rev Inst Med Trop Sao Paulo. 2013 Mar-Apr;55(2):79-83.
24: Ning TZ, Kin WW, Noordin R, Cun ST, Chong FP, Mohamed Z, Olivos-Garcia A, Huat LB. Evaluation of Entamoeba histolytica recombinant phosphoglucomutase protein for serodiagnosis of amoebic liver abscess. BMC Infect Dis. 2013 Mar 21;13:144. doi: 10.1186/1471-2334-13-144.
25: Norsyahida A, Riazi M, Sadjjadi SM, Muhammad Hafiznur Y, Low HC, Zeehaida M, Noordin R. Laboratory detection of strongyloidiasis: IgG-, IgG4 - and IgE-ELISAs and cross-reactivity with lymphatic filariasis. Parasite Immunol. 2013 May-Jun;35(5-6):174-9. doi: 10.1111/pim.12029.
26: Moghadam ZK, Ghaffarifar F, Khalilpour A, Abdul Aziz F, Saadatnia G, Noordin R. IgG4 detection of Echinococcus granulosus paramyosin is a useful diagnostic test for human hydatidosis. Clin Vaccine Immunol. 2013 Apr;20(4):501-5. doi:10.1128/CVI.00019-13.
27: Amerizadeh A, Idris ZM, Khoo BY, Kotresha D, Yunus MH, Karim IZ, Saadatnia G, Teh AY, Noordin R. Identification of Toxoplasma gondii in-vivo induced antigens by cDNA library immunoscreening with chronic toxoplasmosis sera. Microb Pathog. 2013 Jan;54:60-6. doi: 10.1016/j.micpath.2012.09.006.
28: Basuni M, Mohamed Z, Ahmad M, Zakaria NZ, Noordin R. Detection of selected intestinal helminths and protozoa at Hospital Universiti Sains Malaysia using multiplex real-time PCR. Trop Biomed. 2012 Sep;29(3):434-42.
29: Rahumatullah A, Khoo BY, Noordin R. Triplex PCR using new primers for the detection of Toxoplasma gondii. Exp Parasitol. 2012 Jun;131(2):231-8. doi: 10.1016/j.exppara.2012.04.009. Epub 2012 Apr 25.
30: Kotresha D, Poonam D, Muhammad Hafiznur Y, Saadatnia G, Nurulhasanah O, Sabariah O, Tan SY, Izzati Zahidah AK, Rahmah N. Recombinant proteins from new constructs of SAG1 and GRA7 sequences and their usefulness to detect acute toxoplasmosis. Trop Biomed. 2012 Mar;29(1):129-37.
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